human hepatocyte and hepatoma cell lines  (National Centre for Cell Science)

Journal: Annals of Biomedical Engineering

Article Title: An Integrated In Vitro–In Silico Approach for Silver Nanoparticle Dosimetry in Cell Cultures

doi: 10.1007/s10439-020-02449-5

Figure Lengend Snippet: HUVEC (gray line) and C3A (black line) viability as a function of increasing target cell doses. Panels show the viability as measured by the Alamar blue assay at 6 h ( a ), 16 h ( b ) and 24 h ( c ). Data shown as mean ± std ( n = 4).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) and the human hepatoma-derived hepatocyte C3A cell line (ATCC Culture, USA) were used for evaluating nanotoxicity.

Techniques: Alamar Blue Assay

Journal: The FASEB Journal

Article Title: In vivo selective and distant killing of cancer cells using adenovirus-mediated decorin gene transfer

doi: 10.1096/fj.02-0534fje

Figure Lengend Snippet: Evidence for a major local and distant antitumoral effect in vivo, using Ad-mediated decorin gene transfer. Nude mice were injected sc with human lung A549 or human liver Hep3B xenograft cells. When tumors reached volume >50 mm3, an Ad vector encoding human decorin cDNA (Ad.DCN), a control vector (Ad.null), or phophate buffer saline (PBS) was injected in vivo (arrows) intratumorally (graphs I, III, IV) or intravenously (graph II). In graph III, 2 distinct xenografts were concomitantly established (one on each flank), and only one tumor was injected with the Ad vector (intratumoral, IT) whereas the contralateral (CL) tumor remained uninjected.

Article Snippet: The human lung cancer cell lines A549 (ATCC, CCL 185) and H1299 (ATCC CRL-5803), the human hepatocyte cancer cell lines Hep3B (ATCC, HB 8064), HUH7, and HepG2 (ATCC HB-8065), the human colorectal adenocarcinoma cell line Caco-2 (ATCC CRL-2102), and the human cervical adenocarcinoma cell line HeLa (ATCC CCL-2) were maintained in Dulbecco’s modified Eagle’s medium (Gibco-BRL) supplemented with 10% FBS, or as described ( 6 – 8 ).

Techniques: In Vivo, Injection, Plasmid Preparation, Control, Saline

Journal: The FASEB Journal

Article Title: In vivo selective and distant killing of cancer cells using adenovirus-mediated decorin gene transfer

doi: 10.1096/fj.02-0534fje

Figure Lengend Snippet: In vitro selective killing of tumoral cells, using Ad-mediated decorin gene transfer. Human lung A549, H1299 tumor cells, human liver Hep3B, HUH7, HepG2 tumor cells, human colorectal adenocarcinoma Caco-2 cells, human cervical adenocarcinoma HeLa tumor cells, human hepatocytes in primary culture, human umbilical vein endothelial cells (HUVEC), and human fibroblasts were incubated in vitro with an Ad vector encoding human decorin cDNA (Ad.DCN) or a control vector (Ad.null) at different MOI. A). Cell proliferation was measured overtime using Alamar Blue Assay. B). Facs analysis of cell cycle, 6 days after in vitro incubation with Ad vectors. The arrow indicates a peak of cells in sub-G1 phase. The top right panel shows A549 cells incubated with 300 μg/ml purified decorin. C). Northern analysis using human decorin, human p21, and human GAPDH cDNA probes, 2 days after in vitro incubation with Ad vectors. p21/GAPDH is the ratio between p21 and GAPDH mRNAs quantified by Phosphorimager. D). Quantitation of caspase-8 activity in whole cells lysates, 4 days after in vitro Ad.DCN or Ad.null transduction.

Article Snippet: The human lung cancer cell lines A549 (ATCC, CCL 185) and H1299 (ATCC CRL-5803), the human hepatocyte cancer cell lines Hep3B (ATCC, HB 8064), HUH7, and HepG2 (ATCC HB-8065), the human colorectal adenocarcinoma cell line Caco-2 (ATCC CRL-2102), and the human cervical adenocarcinoma cell line HeLa (ATCC CCL-2) were maintained in Dulbecco’s modified Eagle’s medium (Gibco-BRL) supplemented with 10% FBS, or as described ( 6 – 8 ).

Techniques: In Vitro, Incubation, Plasmid Preparation, Control, Alamar Blue Assay, Purification, Northern Blot, Quantitation Assay, Activity Assay, Transduction